電噴霧-質(zhì)譜研究A環(huán)不同取代基對(duì)黃酮類化合物裂解途徑的影響
摘 要：選擇槲皮素、葛根素、黃芩苷、朝藿定C和野黃芩苷為研究對(duì)象，在負(fù)離子檢測(cè)方式下，采用電噴霧-質(zhì)譜法（ESI-MS）研究A環(huán)不同取代基對(duì)自身裂解途徑的影響。結(jié)果發(fā)現(xiàn)，該類化合物主要發(fā)生了脫糖、脫CO、脫水和RDA反應(yīng)，進(jìn)一步分析發(fā)現(xiàn)，A環(huán)上不同的取代基對(duì)自身質(zhì)譜裂解途經(jīng)影響較大，其裂解途徑有顯著差異，且H2O分子的丟失可以成為判斷A環(huán)取代基信息的一種方法。A環(huán)取代基不同黃酮類化合物的質(zhì)譜裂解規(guī)律有助于復(fù)雜黃酮類化合物的快速檢測(cè)和結(jié)構(gòu)解析。
關(guān)鍵詞：電噴霧-質(zhì)譜；黃酮類化合物；裂解特征；RDA裂解
中圖分類號(hào)：TQ406.7+2；TQ463+.24；O657.63；R927.2 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1674-5124（2013）05-0059-03
Study on different substituents in A-ring effect on fragmentation pathway of
flavonoids using electrospray ion trap mass spectrometry
LIN Hong-ying1， HUANG Jian-mei1， DUAN Tian-xuan1， TAN Peng1， LIU Yong-gang1， LI Jiang-ning2
（1. Beijing University of Chinese Medicine，Beijing 100102，China；
2. Beijing Drug Administration，Beijing 100053，China）
Abstract: Under negative ion mode， electrospray ionization mass spectrometry （ESI-MS） was used to study on different substituents in A-ring effect on fragmentation pathway of flavonoids. During the fragmentation， RDA cleavage was detected and CO and H2O were usually lost during the fragmentation. Different substituents in A-ring affected fragmentation obviously， and among them， the loss of H2O molecular could be a marker to judge the substituent in A-ring. The results will be helpful in identifying the structures of flavonoids rapidly with the method of mass spectrometry.
Key words: ESI-MS； flavonoids； fragmentation； RDA cleavage
